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JKMRS Volume 25, No 2, pp 12, Comparison of metabolic profilin...
2021년 06월 20일 / 조회수: 101

Comparison of metabolic profiling of Daphnia magnabetween HR-MAS NMR and solution NMR techniques

 

 

Seonghye Kim, Sujin Lee, Wonho Lee, Yujin Lee, Juyoung Choi, Hani Lee, Youzhen Li, Seulbin Ha, Suhkmann Kim*

 

 

Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University, Busan 46241, Republic of Korea 

 

 

Received June 16, 2021; Revised June 18, 2021; Accepted June 18, 2021

 

Abstract Daphnia magna is used as target organism for environmental metabolomics. The metabolome of D. magna was studied with NMR spectroscopy. Most studies used the extract of D. magna, but the reproducibility cannot be obtained using extracted sample. In this study, lyophilized D. magna samples were analyzed with two different 1H NMR techniques, HR-MAS on intact tissues and solution NMR on extracted tissues. Samples were measured three times using 600 MHz NMR spectrometer. Metabolite extraction required more than twice as many D. magna, but the metabolite intensity was lower in solution NMR. In the spectra of HR-MAS NMR, the lipid signal was observed, but they did not interfere with metabolite profiling. We also confirmed the effect of swelling time on signal intensities of metabolites in HR-MAS NMR, and the results suggest that appropriate swelling should be used in lyophilized D. magna to improve the accuracy of metabolite profiles.

 

Keywords Daphnia magna, Metabolomics, Nuclear magnetic resonance (NMR), High resolution magic angle spinning (HR-MAS) 


* Address correspondence to: Suhkmann Kim, Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University, Busan 46241, Republic of Korea, Tel: 82-51-510-2240; Fax: 82-51-516-7421; E-mail: suhkmann@pusan.ac.kr  

 

첨부파일 1_KimSM.pdf